The Lung Health Research Group at the University of Calgary in the Snyder Institute for Chronic Diseases is involved in numerous research areas targeting aspects of Asthma and COPD for which current therapies are less than ideal. The group is well positioned to study these complex diseases from a number of different perspectives, including studies of inflammatory mechanisms, surfactant function, airway remodeling, and epithelial cell biology, and can test pharmacological interventions in both pre-clinical and clinical settings. This research has been facilitated by the recent opening of a new ~1500 square foot "state-of-the-art" dedicated clinical research suite that is unique in Canada. The group is made up of basic and clinician scientists who are well published and recognized worldwide in their areas of expertise. The collaborative atmosphere within the group promotes a team effort to elucidating the underlying mechanisms of these diseases and finding better therapeutics for combating these ailments.
Airways Inflammation Models
The research group has the capacity to conduct studies at all levels from cell cultures to clinical trials.
Model systems include:
- Cell lines: A variety of human bronchial and alveolar epithelial cell lines are available including: BEAS-2B, 16-HBE, HBE-1 and A549.
- Primary cultures (from normal human lungs):
- Epithelium: can be grown in submersion culture or at air-liquid interface
- Smooth muscle cells (SMCs)
Have initiated co-culture models for epithelial-fibroblast or epithelial-SMC interactions.
Group members also have experience with a variety of other cell types, including macrophages, dendritic cells, neutrophils, eosinophils and mast cells.
- Mouse models of: Allergen challenge (Both Ovalbumin & House Dust Mite models)
- SR antigen model of hypersensitivity pneumonitis (Farmer's lung). Have expertise in fibrosis in this model.
- Acute Lung Injury
- Also have a rat model of: Ovalbumin challenge
There are full facilities for nasal and pulmonary provocation studies, bronchoscopy (lavage/brushing/biopsy), sputum induction, pulmonary function (spirometry & plethysmography), exhaled nitric oxide measurements and exercise testing.
- Allergen challenge - lung/nose
- Experimental rhinovirus infections (soon)
- Studies in asthmatics and patients with COPD
- Interventional bronchoscopy
- Mark Giembycz (Group Chair)
Research Areas: Pulmonary pharmacology, cyclic nucleotide signaling, actions of b-adrenergic agonists, phosphosdiesterase inhibitors, glucocorticoids
- David Proud
Research Areas: Epithelial cell biology, viral exacerbations of asthma and COPD, interactions of viruses and cigarette smoke
- Matthias Amrein
Research areas: Surfactant structure and function, electron/atomic force microscopy
- Alex Chee
Research Areas: Interventional Pulmonary Medicine
- Stephen Field
Research areas: Clinical studies in asthma and COPD
- Francis Green
Research Areas: Pathology of asthma, animal models of asthma, surfactant function
- Margaret Kelly
Research areas: Mechanisms of remodeling and pulmonary fibrosis. Lung pathology
- Richard Leigh
Research areas: Mechanisms of airway remodeling, clinical studies in asthma and COPD
- Robert Newton
Research areas: Molecular biology, mechanisms of glucocorticoid action, mechanisms of glucorticoid/LABA synergy
- Alain Tremblay
Research areas: Interventional bronchoscopy
- Brent Winston
Research areas: Acute lung injury, critical care medicine