Imaging

Type: Inverted Confocal Fluorescence

Modalities: 4-channel fluorescence confocal (Resonant or Galvano scanning), 32-channel spectral confocal (Galvano scanning), transmission and DIC

Special Features: 

• Resonant scanner – 30 frames per second full frame up to 100’s of frames per second for smaller areas, also allows rapid acquisition of large volumes or observation of rapid live events
• Spectral detector – 32 channel visible spectrum scanning for samples with complex emission profiles, super-multichannel imaging
• Hybrid scanning utilizing Galvo scanners to stimulate an area while imaging at high speed with Resonant scanners
• Perfect Focus automatic focus lock system for long-term imaging
• Wide variety of objective lenses available from long-working distance for thick samples to high resolution oil lenses for cells and thin sections
• Continuously variable pinhole – can balance signal vs. resolution for dim or thick samples
• Stage-top incubator system for live samples including temperature and CO2 control
• Automated tiling for large area samples
• Transmitted Laser absorption and DIC imaging
• Low laser exposure for less photobleaching/photodamage
• Free downloadable Nikon Elements Viewer for data review and 3D reconstruction

Optimized for:

• Live samples (cells, organoids, tissue)
• New sample exploration, dye screening, protocol development
• Organoids, thick tissue
• Spectral imaging for autofluorescence reduction and overlapping fluorophores
• Large area/volume acquisition

Technical Specifications:

• Point scan confocal 15-420 FPS (Resonant), up to 10 FPS (Galvano)
• 2k x 2k resolution (Galvo), 1k x 1k resolution (Resonant)
• 405, 488, 561, 640nm laser lines
• High sensitivity GaAsP detectors (Blue/Green), extended red sensitivity detectors (Red/Far Red)
   

Type: Articulated Upright Multi-Axis in-vivo Multiphoton Microscope

Modalities: Two-photon (2P), Second harmonic generation (SHG)

Special Features: 

• Articulated body can rotate +/-45 degrees around sample, capable of traveling over large regions for imaging large samples or areas, or addressing multiple samples in an array
• Resonant scanner – 30 frames per second full frame up to 100’s of frames per second for smaller areas, also allows rapid acquisition of large volumes or observation of rapid live events
• Adaptable stage for large or uniquely-shaped samples, whole live animal support
• Semi-automated power compensation for deep tissue imaging for photodamage reduction and improved depth penetration
• Fast-tuning Ti:Saph laser can switch wavelengths quickly during an experiment
• Four-channel GaAsP detector array for Blue/Green/Red/Far Red fluorophores or SHG
• Dipping objective lenses optimized for thick samples such as organoids, tissue, spheroids, and aggregates
• Automated tiling for large area imaging, 3D point visiting

Optimized for:

• Thick samples (organoids, tissue, organs)
• Cleared samples
• SHG imaging
• Live animal imaging

Technical Specifications:

• Point Scan Multiphoton 2-200 FPS (Resonant, dependent on resolution)
• Articulated rotation body, +/-45 degree rotation, 50x50x50mm XYZ range
• Maximum 4k x 4k resolution, 1k x 1k typical
• 720-1050nm laser tuning range
• High Sensitivity GaAsP detectors (Blue/Green/Red/Far Red)

Type: In-Incubator phase and 2-color fluorescence microscope 

Modalities: Fluorescence and Phase Contrast imaging of well plates. 

Special Features: 

• Incubated - maintains living samples at 37C with 5% CO2 

• Two-color Green/Red fluorescence plus HD Phase imaging 

• Long term imaging of multiple well plates 

 Optimized for: 

• Short/Medium/Long-term Live observation 

• Phase/Brightfield 

• Fluorescence 

• Organoids 
• Scratch Wound 
• Tiled FOV 
• Chemotaxis 
• Angiogenesis 

Technical Specifications: 

• Magnification:  up to 20x 
• Image Resolution:  0.61 microns/pixel (objective lens dependent) 
• Image Format:  1392 x 1040 pixels 
• Field of View:  0.85 x 0.63 mm (objective lens dependent) 
• Fluorescence: Red, Green 

• Phase Contrast 

Type: In-Incubator Fluorescence Microscopy 

Modalities: Fluorescence and Enhanced Widefield imaging of well plates. 

Special Features: 

• Incubated - maintains living samples at 37C with 5% CO2 

• Three-color Blue/Green/Red fluorescence and brightfield imaging 

• Long term imaging of multiple well plates 

Optimized for: 

• Short/Medium/Long-term Live observation 

• Fluorescence 

• Widefield enhanced contrast 
• Organoids 
• Scratch Wound 
• Tiled FOV 
• Chemotaxis 
• Angiogenesis 

Technical Specifications: 

• Magnification: 4X or 10X 
• Image Resolution of 4X: 0.862 μm/pixel 
• Field of View of 4X: 2.1 x 1.77 mm 
• Image Resolution of 10X: 0.345 μm/pixel 
• Field of View of 10X: 0.8 x 0.7 mm 
• Camera Format: 2448 X 2048 pixels 

• Channel blue (Ex 370-410 nm, Em 429-462 nm) 

• Channel green (Ex 473-491 nm, Em 502-561 nm) 
• Channel red (Ex 580-598 nm, Em 612-680 nm) 

Special Features:  

• Leica DMI6000 B Inverted research level microscope with Adaptive Focus Control (AFC) and all fully motorized components including XY stage, piezo Z stage insert.  

• Four different pinhole sizes for spinning disk t 

• Equipped with differential interference contrast (DIC)  

• Multi-modal optical head that is configured for Total Internal Reflection Fluorescence (TIRF), fast live-cell spinning disk confocal imaging and super-resolution.  

• MetaMorph Super-Resolution System supports localization microscopy: PALM, STORM and dSTORM super-resolution techniques.  

• Live cell instrumentation includes an advanced environmental system with CO2, heat and humidity control.  

Optimized for:  

• TIRF, spinning disk and point localization microscopy 

• Multi-color, 3D reconstruction and high-speed time-lapse.  

• Live-cell microscopy 

Technical Specifications:  

• 4 lasers for fluorescence excitation (405nm, 488nm, 561nm, and 637nm) and LED system for widefield fluorescence 

• 2 Andor iXon Ultra 897 back-illuminated EMCCD cameras for simultaneous 2 color imaging as well as high resolution and low light scientific applications 

Type: Intravital microscopy 

Modalities:  Intravital imaging 

Special Features: 

• Fully automated objective nosepiece, reflector turret and light path control 

• Motorized stage (x, y)  

• Motorized focus control (z) 

• Environmental chamber control (temperature, CO2 and humidity) 

Optimized for: 

• Brightfield, DIC and epifluorescence 

• Live cell imaging 

• Confocal optical sectioning 

• Multi-channel imaging 

• Timelapse 

• Z-stack 

• Tile scanning 

• FRAP, FLIP, photo-conversion (405nm laser only) 

• 3D d-STORM 

Technical Specifications: 

• 4 laser lines: 405nm, 490nm, 561nm, 639nm 

• Blue (Ex 360/20nm, FT 400nm, Em 425nm) 

• Green (Ex 470/40nm, FT 510nm, Em 515nm) 

• Red (538/44nm, FT 580nm, Em 590) 

• Blue, green and red confocal filters  

• EM-CCD camera: Hamamatsu EM-CCD ImagEM (512x512) 

• sCMOS camera: Photometrics Prime BSI (1966x1966) [95% QE, back illuminated, 80fps] 

Type: White-light stereoscopic imaging 

Modalities: Stereomicroscope 

Special Features: 

• 1X and 2X lenses 

• UC50 Olympus colour camera  

• White lamp illuminator 

Optimized for: 

• Sample preparation 

• Surgical and sample manipulation 

• Inspection 

Technical Specifications: 

• 1X and 2X lenses 

• White lamp gooseneck illumination 

Type: Upright widefield with colour camera 

Modalities: White-light and polarized imaging 

Special Features: 

• 5 MP QColor 5 Olympus colour camera 

• Crossed Polarized imaging 

Optimized for: 

• Slide mounted samples 

• Birefringent samples (Polarization mode)  

Technical Specifications: 

• 4x/10x/20x/40x objective lenses 

• Crossed polarizer set for polarized imaging 

• 5.0 Megapixel color camera for brightfield or polarization imaging