Imaging

Type: Inverted Confocal Fluorescence

Modalities: 4-channel fluorescence confocal (Resonant or Galvano scanning), 32-channel spectral confocal (Galvano scanning), transmission and DIC

Special Features: 

• Resonant scanner – 30 frames per second full frame up to 100’s of frames per second for smaller areas, also allows rapid acquisition of large volumes or observation of rapid live events
• Spectral detector – 32 channel visible spectrum scanning for samples with complex emission profiles, super-multichannel imaging
• Hybrid scanning utilizing Galvo scanners to stimulate an area while imaging at high speed with Resonant scanners
• Perfect Focus automatic focus lock system for long-term imaging
• Wide variety of objective lenses available from long-working distance for thick samples to high resolution oil lenses for cells and thin sections
• Continuously variable pinhole – can balance signal vs. resolution for dim or thick samples
• Stage-top incubator system for live samples including temperature and CO2 control
• Automated tiling for large area samples
• Transmitted Laser absorption and DIC imaging
• Low laser exposure for less photobleaching/photodamage
• Free downloadable Nikon Elements Viewer for data review and 3D reconstruction

Optimized for:

• Live samples (cells, organoids, tissue)
• New sample exploration, dye screening, protocol development
• Organoids, thick tissue
• Spectral imaging for autofluorescence reduction and overlapping fluorophores
• Large area/volume acquisition

Technical Specifications:

• Point scan confocal 15-420 FPS (Resonant), up to 10 FPS (Galvano)
• 2k x 2k resolution (Galvo), 1k x 1k resolution (Resonant)
• 405, 488, 561, 640nm laser lines
• High sensitivity GaAsP detectors (Blue/Green), extended red sensitivity detectors (Red/Far Red)

Type: High Throughput and Live Confocal Fluorescence with enhanced Far Red detection

Modalities: 5 channel simultaneous (4 fluorescence + 1 transmitted light), 9 channel two-phase, 7 laser excitation with adjustable detection windows for multiple fluorophores, multiple far-red excitation and detection channels in addition to standard visible channels.

Special Features:

• Resonant scanner - 30 frames per second full frame to 100's of frames per second for smaller areas
• Advanced area overview and tiling - features low magnification rapid previews of large area samples followed by higher magnification sub regions for greater detail in relevant areas
• Zero Drift focus system for long-term imaging
• Stage incubator for live samples
• Supports well plates, slides, dishes
• Long working distance objective lenses for slice and organoid imaging, transwells
• High dynamic range detectors for working with samples with large contrast differences
• Free downloadable OlyVIA viewer

Optimized for:

• Live samples (3D and 2D organoids, monolayers, tissue)
• Thick samples using far red fluorophores to reduce light scattering
• Large area/volume acquisition
• Time lapse imaging
• Many-color imaging 7+ channels depending on fluorophore selection

Technical Specifications:

• Point scan confocal 10-400 FPS (Resonant), up to 10 FPS (Galvano)
• 2k x 2k resolution (Galvo), 1k x 1k resolution (Resonant)
• 405, 488, 561, 640, 680, 730, 785nm laser lines
• 2x wide-area survey lens, 4x, 10x, 20x 0.7 NA LWD correction collar lens, 30x 1.05 NA silicone oil lens, 60x 1.5 NA oil lens
• High sensitivity, high dynamic range hybrid detectors, two optimized for visible, two optimized for red/near-IR wavelength ranges
• 20x 0.7NA long working distance lens with correction collar for thick samples
• 30x silicone oil lens for 3D and 2.5D organoid imaging
• 60x precision oil lens for cell culture

Special Features:

• Incubated imaging chamber for multi-day long-term imaging
• Autofocus to maintain focus during long acquisitions
• Supports well plates, slides, dishes
• Long working distance 20x lens with motorized correction collar for organoid imaging
• High-resolution air lenses for imaging cell culture, 2D, 2.5D organoids, and tissue
• Visible and extended red fluorescence channels for up to 5 color fluorescence imaging
• Free downloadable OlyVIA viewer

Optimized for:

• Live samples (3D and 2D organoid) over time
• Slide scanning, up to four slides per run
• Multi-day live experiments
• Wide area/high resolution widefield imaging

Technical Specifications:

• ORCA-Fusion Gen-III sCMOS Camera, low read noise 2k x 2k resolution
• Fluorescence filter sets: DAPI, EGFP/FITC, mCherry/TxRed, Cy5, Cy7
• Gradient contrast and brightfield
• 4x, 10x, 20x 0.7 NA LWD motorized correction collar, 40x 0.95 NA

Modalities: Phase contrast and two or three channel fluorescence widefield imaging

Special Features:

• Up to six well plates simultaneously
• Handles 6 to 96 well plates
• Integrated basic assays

Optimized for:

• Wide area time-lapse imaging, point visiting small area time-lapse imaging
• Well plates, flasks
• Multi-day live experiments
• Phase contrast imaging
• Multi-channel fluorescence imaging

Technical Specifications:

• Three-channel fluorescence module (Green, Orange, Far-Red)
• Two-channel fluorescence module (Green, Red)
• 4x, 10x, 20x objective lenses

Type: Intravital microscopy 

Modalities:  Intravital imaging 

Special Features: 

• Fully automated objective nosepiece, reflector turret and light path control 

• Motorized stage (x, y)  

• Motorized focus control (z) 

• Environmental chamber control (temperature, CO2 and humidity) 

Optimized for: 

• Brightfield, DIC and epifluorescence 

• Live cell imaging 

• Confocal optical sectioning 

• Multi-channel imaging 

• Timelapse 

• Z-stack 

• Tile scanning 

• FRAP, FLIP, photo-conversion (405nm laser only) 

• 3D d-STORM 

Technical Specifications: 

• 4 laser lines: 405nm, 490nm, 561nm, 639nm 

• Blue (Ex 360/20nm, FT 400nm, Em 425nm) 

• Green (Ex 470/40nm, FT 510nm, Em 515nm) 

• Red (538/44nm, FT 580nm, Em 590) 

• Blue, green and red confocal filters  

• EM-CCD camera: Hamamatsu EM-CCD ImagEM (512x512) 

• sCMOS camera: Photometrics Prime BSI (1966x1966) [95% QE, back illuminated, 80fps] 

Type: Articulated Upright Multi-Axis in-vivo Multiphoton Microscope

Modalities: Two-photon (2P), Second harmonic generation (SHG)

Special Features: 

• Articulated body can rotate +/-45 degrees around sample, capable of traveling over large regions for imaging large samples or areas, or addressing multiple samples in an array
• Resonant scanner – 30 frames per second full frame up to 100’s of frames per second for smaller areas, also allows rapid acquisition of large volumes or observation of rapid live events
• Adaptable stage for large or uniquely-shaped samples, whole live animal support
• Semi-automated power compensation for deep tissue imaging for photodamage reduction and improved depth penetration
• Fast-tuning Ti:Saph laser can switch wavelengths quickly during an experiment
• Four-channel GaAsP detector array for Blue/Green/Red/Far Red fluorophores or SHG
• Dipping objective lenses optimized for thick samples such as organoids, tissue, spheroids, and aggregates
• Automated tiling for large area imaging, 3D point visiting

Optimized for:

• Thick samples (organoids, tissue, organs)
• Cleared samples
• SHG imaging
• Live animal imaging

Technical Specifications:

• Point Scan Multiphoton 2-200 FPS (Resonant, dependent on resolution)
• Articulated rotation body, +/-45 degree rotation, 50x50x50mm XYZ range
• Maximum 4k x 4k resolution, 1k x 1k typical
• 720-1050nm laser tuning range
• High Sensitivity GaAsP detectors (Blue/Green/Red/Far Red)

Modalities: STimulated-Emission Depletion (STED), confocal

Special Features:

• 30nm resolution
• 4-channel fluorescence Confocal/STED

Optimized for:

• Fluorescent samples labeled with STED-compatible fluorophores
• Fixed cells and tissue

Technical Specifications:

• 405, 488, 561, 640 nm excitation lasers
• 775 nm depletion laser
• 100x 1.45 NA oil lens

Modalities: Single-molecule localisation microscopy (SMLM) / Stochastic Optical Reconstruction Microscopy (STORM), WF/TIRF/HILO

Special Features:

• 20nm resolution
• Total Internal ReFlection (TIRF) and HILO illumination

Optimized for:

• Extracellular vesicles (EVs)
• Single-molecule localisation

Technical Specifications:

• 405, 488, 561, 640 nm excitation
• 20 nm XY, 50 nm Z resolution (astigmatism deconvolution)
• Simultaneous 2-color imaging
• 50 x 80 um field of view
• NIR autofocus laser
• 100 frames per second camera acquisition

Type: Upright widefield with colour camera 

Modalities: White-light and polarized imaging 

Special Features: 

• 5 MP QColor 5 Olympus colour camera 

• Crossed Polarized imaging 

Optimized for: 

• Slide mounted samples 

• Birefringent samples (Polarization mode)  

Technical Specifications: 

• 4x/10x/20x/40x objective lenses 

• Crossed polarizer set for polarized imaging 

• 5.0 Megapixel color camera for brightfield or polarization imaging 

Details coming soon!

Modalities: Fluorescence widefield, Total Internal ReFlection (TIRF)

Special Features:

• Seven-channel fluorescence widefield imaging
• Total Internal ReFlection (TIRF) imaging
• Stage-top incubator for long-term imaging

Optimized for:

• Cell culture
• Well plates, dishes, slides

Technical Specifications:

• 7-color LED excitation source (395, 440, 470, 510, 550, 575, 640 nm)
• 4-color TIRF laser excitation source (405, 488, 561, 640 nm)
• Hamamatsu Orca flash 4.0 v2 sCMOS 16-bit camera