Imaging

The LCI provides access to optical imaging equipment, from sample preparation to image analysis. We also provide design services and collaboration for imaging technology and devices.

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Ambreen Akhtar

Nikon A1R+ Confocal Microscope

Type: Inverted Confocal Fluorescence

Modalities: 4-channel fluorescence confocal (Resonant or Galvano scanning), 32-channel spectral confocal (Galvano scanning), transmission and DIC

Special Features:

Resonant scanner – 30 frames per second full frame up to 100’s of frames per second for smaller areas, also allows rapid acquisition of large volumes or observation of rapid live events
Spectral detector – 32 channel visible spectrum scanning for samples with complex emission profiles, super-multichannel imaging
Hybrid scanning utilizing Galvo scanners to stimulate an area while imaging at high speed with Resonant scanners
Perfect Focus automatic focus lock system for long-term imaging
Wide variety of objective lenses available from long-working distance for thick samples to high resolution oil lenses for cells and thin sections
Continuously variable pinhole – can balance signal vs. resolution for dim or thick samples
Stage-top incubator system for live samples including temperature and CO2 control
Automated tiling for large area samples
Transmitted Laser absorption and DIC imaging
Low laser exposure for less photobleaching/photodamage
Free downloadable Nikon Elements Viewer for data review and 3D reconstruction

Optimized for:

Live samples (cells, organoids, tissue)
New sample exploration, dye screening, protocol development
Organoids, thick tissue
Spectral imaging for autofluorescence reduction and overlapping fluorophores
Large area/volume acquisition

Technical Specifications:

Point scan confocal 15-420 FPS (Resonant), up to 10 FPS (Galvano)
2k x 2k resolution (Galvo), 1k x 1k resolution (Resonant)
405, 488, 561, 640nm laser lines
High sensitivity GaAsP detectors (Blue/Green), extended red sensitivity detectors (Red/Far Red)

Ambreen Akhtar

Bergamo Multiphoton Microscope

Type: Articulated Upright Multi-Axis in-vivo Multiphoton Microscope

Modalities: Two-photon (2P), Second harmonic generation (SHG)

Special Features:

Articulated body can rotate +/-45 degrees around sample, capable of traveling over large regions for imaging large samples or areas, or addressing multiple samples in an array
Resonant scanner – 30 frames per second full frame up to 100’s of frames per second for smaller areas, also allows rapid acquisition of large volumes or observation of rapid live events
Adaptable stage for large or uniquely-shaped samples, whole live animal support
Semi-automated power compensation for deep tissue imaging for photodamage reduction and improved depth penetration
Fast-tuning Ti:Saph laser can switch wavelengths quickly during an experiment
Four-channel GaAsP detector array for Blue/Green/Red/Far Red fluorophores or SHG
Dipping objective lenses optimized for thick samples such as organoids, tissue, spheroids, and aggregates
Automated tiling for large area imaging, 3D point visiting

Optimized for:

Thick samples (organoids, tissue, organs)
Cleared samples
SHG imaging
Live animal imaging

Technical Specifications:

Point Scan Multiphoton 2-200 FPS (Resonant, dependent on resolution)
Articulated rotation body, +/-45 degree rotation, 50x50x50mm XYZ range
Maximum 4k x 4k resolution, 1k x 1k typical
720-1050nm laser tuning range
High Sensitivity GaAsP detectors (Blue/Green/Red/Far Red)

Incucyte® SX1 Live-Cell Analysis System

Type: In-Incubator phase and 2-color fluorescence microscope

Modalities: Fluorescence and Phase Contrast imaging of well plates.

Special Features:

Incubated - maintains living samples at 37C with 5% CO2
Two-color Green/Red fluorescence plus HD Phase imaging
Long term imaging of multiple well plates

Optimized for:

Short/Medium/Long-term Live observation
Phase/Brightfield
Fluorescence
Organoids
Scratch Wound
Tiled FOV
Chemotaxis
Angiogenesis

Technical Specifications:

Magnification: up to 20x
Image Resolution: 0.61 microns/pixel (objective lens dependent)
Image Format: 1392 x 1040 pixels
Field of View: 0.85 x 0.63 mm (objective lens dependent)
Fluorescence: Red, Green
Phase Contrast

CELLCYTE X™ Microscope

Type: In-Incubator Fluorescence Microscopy

Modalities: Fluorescence and Enhanced Widefield imaging of well plates.

Special Features:

Incubated - maintains living samples at 37C with 5% CO2
Three-color Blue/Green/Red fluorescence and brightfield imaging
Long term imaging of multiple well plates

Optimized for:

Short/Medium/Long-term Live observation
Fluorescence
Widefield enhanced contrast
Organoids
Scratch Wound
Tiled FOV
Chemotaxis
Angiogenesis

Technical Specifications:

Magnification: 4X or 10X
Image Resolution of 4X: 0.862 μm/pixel
Field of View of 4X: 2.1 x 1.77 mm
Image Resolution of 10X: 0.345 μm/pixel
Field of View of 10X: 0.8 x 0.7 mm
Camera Format: 2448 X 2048 pixels
Channel blue (Ex 370-410 nm, Em 429-462 nm)
Channel green (Ex 473-491 nm, Em 502-561 nm)
Channel red (Ex 580-598 nm, Em 612-680 nm)

Ambreen Akhtar

Quorum Spinning Disk

Type: Intravital microscopy

Modalities: Intravital imaging

Special Features:

Fully automated objective nosepiece, reflector turret and light path control
Motorized stage (x, y)
Motorized focus control (z)
Environmental chamber control (temperature, CO2 and humidity)

Optimized for:

Brightfield, DIC and epifluorescence
Live cell imaging
Confocal optical sectioning
Multi-channel imaging
Timelapse
Z-stack
Tile scanning
FRAP, FLIP, photo-conversion (405nm laser only)
3D d-STORM

Technical Specifications:

4 laser lines: 405nm, 490nm, 561nm, 639nm
Blue (Ex 360/20nm, FT 400nm, Em 425nm)
Green (Ex 470/40nm, FT 510nm, Em 515nm)
Red (538/44nm, FT 580nm, Em 590)
Blue, green and red confocal filters
EM-CCD camera: Hamamatsu EM-CCD ImagEM (512x512)
sCMOS camera: Photometrics Prime BSI (1966x1966) [95% QE, back illuminated, 80fps]